ODN 2088
| ODN 2088 | Unit size | Cat. code | Docs | Price |
|---|---|---|---|---|
TLR9 antagonist - Mouse preferred | 200 µg 1 mg 5 mg | tlrl-2088 | TDSMSDS | Please contact our distributor Add to favorite |
- About
- Specifications
- Contents
- Citations
TLR9 Antagonist - Inhibitory ODN, preference towards mouse
Toll-like receptor 9 (TLR9) recognizes specific unmethylated CpG oligonucleotide (ODN) sequences that distinguish microbial DNA from mammalian DNA.
Studies suggest the existence of DNA sequences that can neutralize the stimulatory effect of CpG ODNs [1].
These sequences are characterized by three consecutive Gs downstream of a C or A, addition of a fourth G (G-tetrads) increases the inhibitory capability.
The most potent inhibitory sequences are (TTAGGG)4 found in mammalian telomeres [2] and ODN 2088 which derives from a stimulatory ODN by replacement of 3 bases [3].
Inhibitory ODNs act by disrupting the colocalization of CpG ODNs with TLR9 in endosomal vesicles without affecting cellular binding and uptake.
References:
1. Krieg AM. et al., 1998. Sequence motifs in adenoviral DNA block immune activation by stimulatory CpG motifs. Proc Natl Acad Sci USA. 95(21): 12631-6.2. Gursel I. et al., 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.3. Stunz LL. et al., 2002. Inhibitory oligodinucleotides specifically block effects of stimulatory CpG oligonucleotides in B cells. Eur J Immunol. 32(5): 1212-22.
Back to the topSpecifications
Specificity: murine TLR9 antagonist
Working concentration: 100 nM - 10 µM
Solubility: 2.5 mg/ml in water
ODN 2088 sequence5’- tcc tgg cgg gga agt -3’ (15 mer)Note: Bases are phosphorothioate (nuclease resistant).
Quality control:
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Contents
ODN 2088 is provided lyophilized and is available in three quantities:
- 200 μg (41 nmol): tlrl-2088 (formerly tlrl-minhodn)
- 1 mg (205 nmol): tlrl-2088-5 (formerly tlrl-minhodn-5)
- 5 mg (1.025 mmol): tlrl-2088-5 (formerly tlrl-minhodn-5)
- 1.5 ml endotoxin-free water
ODN 2088 is shipped at room temperature.
Upon receipt, store at -20 °C.
Citations
2018 Diabetologia. DOI: 10.1007/s00125-018-4705-0
Toll-like receptor 9 negatively regulates pancreatic islet beta cell growth and function in a mouse model of type 1 diabetes.
Liu M. et al.
2018 Pathol Oncol Res DOI: 10.1007/s12253-018-0544-z
Modified Genomic Self-DNA Influences In Vitro Survival of HT29 Tumor Cells via TLR9- and Autophagy Signaling
Sipos F. et al.
2018 Cells. DOI: 10.3390/cells8010003
Extracellular Histones Activate Plasma Membrane Toll-Like Receptor 9 to Trigger Calcium Oscillations in Rat Pancreatic Acinar Tumor Cell AR4-2J.
Guo H.Y. & Cui Z.J.
2017 Front Immunol. 8:1039.
Type I Interferon Induced by Streptococcus suis Serotype 2 is Strain-Dependent and May Be Beneficial for Host Survival.
Auger JP. et al.
2017 Cell Physiol Biochem. 41(4):1547-1554.
Prevention of TLR9 Pathway in Warm Ischemia in Porcine Donor Liver after Cardiac Death.
Shao Z. et al.
2017 PLoS One. 12(1):e0169245.
Heme Oxygenase-1 Deficiency Diminishes Methicillin-Resistant Staphylococcus aureus Clearance Due to Reduced TLR9 Expression in Pleural Mesothelial Cells.
Gahlot S. et al.
2016 Arthritis Res Ther. 18(1):264.
Circulating exosomes from patients with systemic lupus erythematosus induce an proinflammatory immune response.
Lee JY. et al.
2016 Ann Rheum Dis. 75(10):1858-65.
Nucleosome in patients with systemic sclerosis: possible association with immunological abnormalities via abnormal activation of T and B cells.
Yoshizaki A. et al.
2015 FASEB J. ;29(2):684-95
Gut commensal microvesicles reproduce parent bacterial signals to host immune and enteric nervous systems.
Al-Nedawi K, Mian MF, Hossain N, Karimi K, Mao YK, Forsythe P, Min KK, Stanisz AM, Kunze WA, Bienenstock J.
2014 J Am Heart Assoc.3(2):e000629.
The TLR9 ligand, CpG-ODN, induces protection against cerebral ischemia/reperfusion injury via activation of PI3K/Akt signaling.
Lu C, Ha T, Wang X, Liu L, Zhang X, Kimbrough EO, Sha Z, Guan M, Schweitzer J, Kalbfleisch J, Williams D, Li C.
2011 Blood 117: 6459 - 6468
The genome of self-complementary adeno-associated viral vectors increases Toll-like receptor 9-dependent innate immune responses in the liver.
Martino A.T. et al.
2011 J. Immunol. 186: 6417 - 6426
Painful pathways induced by TLR stimulation of dorsal root ganglion neurons.
Qi J, Buzas K, Fan H, Cohen JI, Wang K, Mont E, Klinman D, Oppenheim JJ, Howard OM
Load more
